Amino acid transport and protein synthesis in induced ectoderm of amphibian embryos

Isolated gastrula ectoderm ofTriturus alpestris orAmbystoma mexicanum was induced by the vegetalizing factor. Protein synthesis in the induced and uninduced control explants was measured by double labelling with H-and C-amino acids after different periods of cultivation. Slight differences were obse...

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Veröffentlicht in:Wilhelm Roux's Archives of Developmental Biology 1979-09, Vol.187 (3), p.211-217
Hauptverfasser: Minuth, Wilhelm, Minuth, Maria, Tiedemann, Heinz
Format: Artikel
Sprache:eng
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Zusammenfassung:Isolated gastrula ectoderm ofTriturus alpestris orAmbystoma mexicanum was induced by the vegetalizing factor. Protein synthesis in the induced and uninduced control explants was measured by double labelling with H-and C-amino acids after different periods of cultivation. Slight differences were observed in the pattern of nuclear proteins after 12 h of cultivation and in the pattern of cytoplasmic proteins after 48 h of cultivation.The uptake of leucine started to increase in induced explants after 48 h of cultivation and after 96 h was about 50 times greater than in uninduced control explants. The uptake is reduced under partially anaerobic conditions. Ouabain inhibits the uptake by about 50%.
ISSN:0340-0794
1432-041X
DOI:10.1007/BF00848617