Glycolytic activity in embryos of Pisum sativum and of non-dormant or dormant seeds of Avena sativa L. expressed through activities of PFK and PPi-PFK

A quantitative cytochemical assay for PPi-PFK activity in the presence of Fru-2,6-P2 is described along with its application to determine levels of activity in embryos of Pisum sativum and Avena sativa. The activity of ATP-PFK has also been studied in parallel as have PFK activities during the switc...

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Veröffentlicht in:	Histochemistry 1989-01, Vol.90 (5), p.359-364
Hauptverfasser:	CORBINEAU, F, CARMIGNAC, D. F, GAHAN, P. B, MAPLE, A. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Animals Biological and medical sciences Economic plant physiology Embryo development. Germination Embryo, Nonmammalian - enzymology Fabaceae - enzymology Fundamental and applied biological sciences. Psychology Growth and development Histocytochemistry - methods Phosphofructokinase-1 - metabolism Phosphotransferases - metabolism Plants - embryology Plants - enzymology Plants, Medicinal Seeds - enzymology
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Zusammenfassung:	A quantitative cytochemical assay for PPi-PFK activity in the presence of Fru-2,6-P2 is described along with its application to determine levels of activity in embryos of Pisum sativum and Avena sativa. The activity of ATP-PFK has also been studied in parallel as have PFK activities during the switch from dormant to non-dormant embryos in Avena sativa. PPi-PFK activity has been demonstrated in all tissues of Pisum sativum embryos and of Avena sativa embryos including the scutellum and the aleurone layers. The PPi-PFK activity was greater than that of ATP-PFK in both dormant and non-dormant seeds though with only marginally more activity in the dormant as opposed to the non-dormant state.
ISSN:	0301-5564 1432-119X
DOI:	10.1007/BF00508313