Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes
We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition o...
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| Veröffentlicht in: | Histochemistry 1986-01, Vol.84 (3), p.279-280 |
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| container_end_page | 280 |
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| container_issue | 3 |
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| container_title | Histochemistry |
| container_volume | 84 |
| creator | ANDERSEN, A. P LYON, H JAKOBSEN, P HOYER, P. E |
| description | We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y. |
| doi_str_mv | 10.1007/BF00495796 |
| format | Article |
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P ; LYON, H ; JAKOBSEN, P ; HOYER, P. E</creator><creatorcontrib>ANDERSEN, A. P ; LYON, H ; JAKOBSEN, P ; HOYER, P. E</creatorcontrib><description>We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.</description><identifier>ISSN: 0301-5564</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/BF00495796</identifier><identifier>PMID: 2423483</identifier><identifier>CODEN: HCMYAL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Formaldehyde ; Fundamental and applied biological sciences. Psychology ; Metabolisms and neurohumoral controls ; Methyl Green ; Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen ; Nucleic Acids - analysis ; Pyronine ; Rats ; Rosaniline Dyes ; Staining and Labeling ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Xanthenes</subject><ispartof>Histochemistry, 1986-01, Vol.84 (3), p.279-280</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c301t-c1f512605e193802b5ba3acb68232901c715ee50dd42468ad41e8e523c4408f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7924098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2423483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ANDERSEN, A. P</creatorcontrib><creatorcontrib>LYON, H</creatorcontrib><creatorcontrib>JAKOBSEN, P</creatorcontrib><creatorcontrib>HOYER, P. E</creatorcontrib><title>Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes</title><title>Histochemistry</title><addtitle>Histochemistry</addtitle><description>We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Formaldehyde</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Methyl Green</subject><subject>Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen</subject><subject>Nucleic Acids - analysis</subject><subject>Pyronine</subject><subject>Rats</subject><subject>Rosaniline Dyes</subject><subject>Staining and Labeling</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Xanthenes</subject><issn>0301-5564</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1EAQhhtR1tnVi3ehD-JBiFZ_Jd3edHFXYdGLgrdQ6VR2WjofdidKbv50MzvDevRURT1PFRQvY88EvBYA1Zv3VwDamcqVD9hOaCULIdz3h2wHCkRhTKkfs_OcfwCUUFX2jJ1JLZW2asf-fF58pOA5-tDyPGMYwnDLf4d5z-c98Z7m_Rr5bSIaimlN44bvhmP7liP3Yz9hCnkc-NjdLSyZDu20JOLtSpnj0B60npIPGOPK8ReGiE088ifsUYcx09NTvWDfrj58vfxY3Hy5_nT57qbw2w9z4UVnhCzBkHDKgmxMgwp9U1qppAPhK2GIDLStlrq02GpBloxUXmuwnVMX7OXx7pTGnwvlue5D9hQjDjQuua5Ka0BY_V9RaCfACbuJr46iT2POibp6SqHHtNYC6kMu9b9cNvn56erS9NTeq6cgNv7ixDF7jF3CwYd8r1VOanBW_QX0WJS6</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>ANDERSEN, A. P</creator><creator>LYON, H</creator><creator>JAKOBSEN, P</creator><creator>HOYER, P. E</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19860101</creationdate><title>Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes</title><author>ANDERSEN, A. P ; LYON, H ; JAKOBSEN, P ; HOYER, P. E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-c1f512605e193802b5ba3acb68232901c715ee50dd42468ad41e8e523c4408f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Formaldehyde</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Methyl Green</topic><topic>Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen</topic><topic>Nucleic Acids - analysis</topic><topic>Pyronine</topic><topic>Rats</topic><topic>Rosaniline Dyes</topic><topic>Staining and Labeling</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ANDERSEN, A. P</creatorcontrib><creatorcontrib>LYON, H</creatorcontrib><creatorcontrib>JAKOBSEN, P</creatorcontrib><creatorcontrib>HOYER, P. E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ANDERSEN, A. P</au><au>LYON, H</au><au>JAKOBSEN, P</au><au>HOYER, P. E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes</atitle><jtitle>Histochemistry</jtitle><addtitle>Histochemistry</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>84</volume><issue>3</issue><spage>279</spage><epage>280</epage><pages>279-280</pages><issn>0301-5564</issn><eissn>1432-119X</eissn><coden>HCMYAL</coden><abstract>We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2423483</pmid><doi>10.1007/BF00495796</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-5564 |
| ispartof | Histochemistry, 1986-01, Vol.84 (3), p.279-280 |
| issn | 0301-5564 1432-119X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Formaldehyde Fundamental and applied biological sciences. Psychology Metabolisms and neurohumoral controls Methyl Green Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen Nucleic Acids - analysis Pyronine Rats Rosaniline Dyes Staining and Labeling Vertebrates: anatomy and physiology, studies on body, several organs or systems Xanthenes |
| title | Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes |
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