Nucleic acid staining with the methyl green-pyronin method: a comparison of the use of pure dyes and commercially available dyes

We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition o...

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Veröffentlicht in:Histochemistry 1986-01, Vol.84 (3), p.279-280
Hauptverfasser: ANDERSEN, A. P, LYON, H, JAKOBSEN, P, HOYER, P. E
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Sprache:eng
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Zusammenfassung:We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00495796