Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein
Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 microM. Low sensitivit...
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| Veröffentlicht in: | Pflügers Archiv 1994-08, Vol.428 (1), p.91-93 |
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| container_title | Pflügers Archiv |
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| creator | GORDIENKO, D. V TSUKAHARA, H |
| description | Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 microM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type. |
| doi_str_mv | 10.1007/bf00374756 |
| format | Article |
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Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/bf00374756</identifier><identifier>PMID: 7971164</identifier><identifier>CODEN: PFLABK</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Arteries - cytology ; Arteries - drug effects ; Arteries - metabolism ; Biological and medical sciences ; Blood vessels and receptors ; Cells, Cultured ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Fundamental and applied biological sciences. 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Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type.</description><subject>Animals</subject><subject>Arteries - cytology</subject><subject>Arteries - drug effects</subject><subject>Arteries - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Ion Channel Gating - drug effects</subject><subject>Kinetics</subject><subject>Membrane Potentials - drug effects</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><subject>Sodium Channels - drug effects</subject><subject>Sodium Channels - metabolism</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Umbilical Veins - cytology</subject><subject>Umbilical Veins - drug effects</subject><subject>Umbilical Veins - metabolism</subject><subject>Vertebrates: cardiovascular system</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UMtOGzEUtSoQTUM33SN5wapowB577GRZImiREN3AOrq27yguHjvyeCLor_RnMSLN6j7OQzqHkG-cXXLG9JXpGRNa6k59IjMuRdu0jIsjMqtv3iitFp_Jl3H8wxhr5aI9ISd6qTlXckb-PWLJyaWSXnxsTEj2GUxA6nCbAmT_F4pPsQFb_A4KOvoAF9ROOWMsI_WRQr1CmXKFMFafDQYPgVoMgQYf352y31W0z2mgGUoVFcwhGcgUcl0pREc30wCRToPxwduq36GPp-S4hzDi1_2ck6fbm8fVr-b-98-71Y_7xgquS2Od0bbGkQxw2XKn-k5IpZHjcqGtrZdQCpe9MIo76LTjNbzo5MJ0rOtZJ-bk-4evzWkcM_brbfYD5Nc1Z-v3gtfXt_8LruSzD_J2MgO6A3XfaMXP9ziMNUifIVo_HmiylZq1WrwBNbSFlQ</recordid><startdate>199408</startdate><enddate>199408</enddate><creator>GORDIENKO, D. 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Psychology</topic><topic>Humans</topic><topic>Ion Channel Gating - drug effects</topic><topic>Kinetics</topic><topic>Membrane Potentials - drug effects</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><topic>Sodium Channels - drug effects</topic><topic>Sodium Channels - metabolism</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Umbilical Veins - cytology</topic><topic>Umbilical Veins - drug effects</topic><topic>Umbilical Veins - metabolism</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GORDIENKO, D. V</creatorcontrib><creatorcontrib>TSUKAHARA, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GORDIENKO, D. V</au><au>TSUKAHARA, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein</atitle><jtitle>Pflügers Archiv</jtitle><addtitle>Pflugers Arch</addtitle><date>1994-08</date><risdate>1994</risdate><volume>428</volume><issue>1</issue><spage>91</spage><epage>93</epage><pages>91-93</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><coden>PFLABK</coden><abstract>Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 microM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>7971164</pmid><doi>10.1007/bf00374756</doi><tpages>3</tpages></addata></record> |
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| ispartof | Pflügers Archiv, 1994-08, Vol.428 (1), p.91-93 |
| issn | 0031-6768 1432-2013 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF00374756 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Arteries - cytology Arteries - drug effects Arteries - metabolism Biological and medical sciences Blood vessels and receptors Cells, Cultured Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Humans Ion Channel Gating - drug effects Kinetics Membrane Potentials - drug effects Patch-Clamp Techniques Rats Sodium Channels - drug effects Sodium Channels - metabolism Tetrodotoxin - pharmacology Umbilical Veins - cytology Umbilical Veins - drug effects Umbilical Veins - metabolism Vertebrates: cardiovascular system |
| title | Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein |
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