Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein

Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 microM. Low sensitivit...

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Veröffentlicht in:	Pflügers Archiv 1994-08, Vol.428 (1), p.91-93
Hauptverfasser:	GORDIENKO, D. V, TSUKAHARA, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arteries - cytology Arteries - drug effects Arteries - metabolism Biological and medical sciences Blood vessels and receptors Cells, Cultured Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Humans Ion Channel Gating - drug effects Kinetics Membrane Potentials - drug effects Patch-Clamp Techniques Rats Sodium Channels - drug effects Sodium Channels - metabolism Tetrodotoxin - pharmacology Umbilical Veins - cytology Umbilical Veins - drug effects Umbilical Veins - metabolism Vertebrates: cardiovascular system
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Zusammenfassung:	Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 microM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type.
ISSN:	0031-6768 1432-2013
DOI:	10.1007/bf00374756