Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesi...

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Veröffentlicht in:	Current genetics 1993-05, Vol.23 (5-6), p.547-548
Hauptverfasser:	Cottarel, G. (Howard Hughes Medical Inst., Cold Spring Harbor, NY (USA). Cold Spring Harbor Lab.), Beach, D, Deuschle, U
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN RECOMBINADO ADN RECOMBINE Autonomously replicating sequence Biological and medical sciences Biotechnology Cloning, Molecular Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies MUTACION Mutagenesis MUTATION Nested deletions Plasmids Recombinant DNA Restriction Mapping Schizosaccharomyces - genetics SCHIZOSACCHAROMYCES POMBE SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE VECTEUR GENETIQUE VECTORES GENETICOS Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:	Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.
ISSN:	0172-8083 1432-0983
DOI:	10.1007/BF00312650