Purification and characterization of invertase from Aspergillus niger

Purification and characterization of invertase from Aspergillus niger

Invertase produced by a strain of Aspergillus niger showed the following main characteristics: maximum activity at 60 degrees C, pH 5.0; Km with sucrose as substrate, 0.0625 mM; Vmax 0.013 mol/min; and free energy 9132 cal/mol. The metal ions and p-chloromercuribenzoate (PCMB) acted as inhibitors re...

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Veröffentlicht in:Current microbiology 1995-08, Vol.31 (2), p.80-83
Hauptverfasser: Rubio, M.C. (National University of Tucuman, Tucuman, Argentina.), Maldonado, M.C
Format: Artikel
Sprache:eng
Schlagworte:
ASPERGILLUS NIGER
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
FRUCTOFURANOSIDASA
FRUCTOFURANOSIDASE
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mission oriented research
Mycology
Physiology and metabolism
PROPIEDADES FISICO-QUIMICAS
PROPRIETE PHYSICOCHIMIQUE
PURIFICACION
PURIFICATION
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Zusammenfassung:Invertase produced by a strain of Aspergillus niger showed the following main characteristics: maximum activity at 60 degrees C, pH 5.0; Km with sucrose as substrate, 0.0625 mM; Vmax 0.013 mol/min; and free energy 9132 cal/mol. The metal ions and p-chloromercuribenzoate (PCMB) acted as inhibitors respectively
ISSN:0343-8651
1432-0991
DOI:10.1007/bf00294280