Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto population (Italy)

Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto population (Italy)

Human red cell Esterase D (EsD) was analyzed by isoelectric focusing (IEF) on ultrathin-layer polyacrylamide gel with a pH range of 5.0-6.0. Hemolysates were treated with Dithiothreitol to avoid loss of activity and change of the isozyme patterns by in vitro storage effects. In our sample of 951 unr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Zeitschrift f r Rechtsmedizin 1987-01, Vol.98 (1), p.39-42
Hauptverfasser: Cortivo, P, Caenazzo, L, Crestani, C, Scoretti, C, Benciolini, P, Ongaro, G
Format: Artikel
Sprache:eng
Schlagworte:
Carboxylesterase
Carboxylic Ester Hydrolases - genetics
Erythrocytes - enzymology
Gene Frequency
Genetics, Population
Humans
Isoenzymes - genetics
Italy
Phenotype
Polymorphism, Genetic
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Human red cell Esterase D (EsD) was analyzed by isoelectric focusing (IEF) on ultrathin-layer polyacrylamide gel with a pH range of 5.0-6.0. Hemolysates were treated with Dithiothreitol to avoid loss of activity and change of the isozyme patterns by in vitro storage effects. In our sample of 951 unrelated persons from Veneto, seven different phenotypes were observed. The following allele frequencies were calculated: EsD1 = 0.8476, EsD2 = 0.1336, EsD5 = 0.0178, and EsDV = 0.0010.
ISSN:0044-3433
1437-1596
DOI:10.1007/BF00200384