Cloning and Characterization ofSOX5,a New Member of the HumanSOXGene Family
The mammalian Y-linked testis determining gene,SRY,encodes a protein with a DNA binding motif known as the HMG box. A large family of genes sharing a high similarity with theSRYHMG box and namedSox(Sry-related HMG box) in mouse andSOXin human has been identified from various organisms. We have clone...
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Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1996-09, Vol.36 (2), p.354-358 |
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Sprache: | eng |
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Zusammenfassung: | The mammalian Y-linked testis determining gene,SRY,encodes a protein with a DNA binding motif known as the HMG box. A large family of genes sharing a high similarity with theSRYHMG box and namedSox(Sry-related HMG box) in mouse andSOXin human has been identified from various organisms. We have clonedSOX5,a new member of the humanSOXgene family.SOX5cDNAs isolated from a human adult testis cDNA library show a high similarity with the mouseSox5transcript over a large region identical in all the human cDNAs. However, comparison of the 5′ unique sequences of the cDNAs suggests that theSOX5gene is subject to alternative splicing. Genomic analysis identified aSOX5pseudogene located on 8q21.1, whereas theSOX5gene itself, which contains a minimum of five introns, maps to 12p12.1. In contrast to the mouse gene, the humanSOX5gene is expressed in a variety of human tissues, and different size transcripts are observed in adult testis and fetal brain. |
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ISSN: | 0888-7543 1089-8646 |
DOI: | 10.1006/geno.1996.0474 |