Assignment of Overlapping 1H NMR Signals in Carp Seminal Plasma by Proton-Detected 2D C,H Correlation Spectroscopy

Assignment of Overlapping 1H NMR Signals in Carp Seminal Plasma by Proton-Detected 2D C,H Correlation Spectroscopy

The 1H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, altho...

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Veröffentlicht in:Biochemical and biophysical research communications 2000-03, Vol.269 (1), p.76-80
Hauptverfasser: Kalic, Marianne, Lauterwein, Jürgen, Büsselmann, Gero, Kamp, Günter
Format: Artikel
Sprache:eng
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Zusammenfassung:The 1H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key proton signals were strongly overlapped.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2250