The Localization of the Site of ArylalkylamineN-Acetyltransferase Circadian Expression in the Photoreceptor Cells of Mammalian Retina

To investigate the molecular mechanism of the melatonin rhythm in the mammalian retina, we examined the temporal mRNA expression pattern of arylalkylamine (serotonin)N-acetyltransferase (AA-NAT), the rate-limiting enzyme in melatonin synthesis in the rat retina. Rat AA-NAT mRNA was detected exclusiv...

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Veröffentlicht in:Biochemical and biophysical research communications 1998-07, Vol.248 (1), p.115-120
Hauptverfasser: Niki, Tomoko, Hamada, Toshiyuki, Ohtomi, Michiko, Sakamoto, Katsuhiko, Suzuki, Satoru, Kako, Koichrou, Hosoya, Yasuhiko, Horikawa, Kazumasa, Ishida, Norio
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Sprache:eng
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Zusammenfassung:To investigate the molecular mechanism of the melatonin rhythm in the mammalian retina, we examined the temporal mRNA expression pattern of arylalkylamine (serotonin)N-acetyltransferase (AA-NAT), the rate-limiting enzyme in melatonin synthesis in the rat retina. Rat AA-NAT mRNA was detected exclusively in the retinal photoreceptors in the outer nuclear layer—low during the day and increased more than threefold at night. The nocturnal AA-NAT expression in rat retina was also confirmed by RNase protection and the AA-NAT enzymatic activity. This is the first report to localize the site of AA-NAT mRNA circadian expression in mammalian photoreceptor cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.8916