Long Time-Scale Probing of the Protein Globular Core Using Hydrogen-Exchange and Room Temperature Phorphorescence

Long Time-Scale Probing of the Protein Globular Core Using Hydrogen-Exchange and Room Temperature Phorphorescence

Preliminary room temperature phosphorescence measurements of the highly buried Trp109 inE. colialkaline phosphatase have been used to report on the kinetics of protein hydrogen–deuterium exchange. Upon dilution in D2O the phosphorescence lifetime increases (at 20°C) in a biphasic manner with an imme...

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Veröffentlicht in:Biochemical and biophysical research communications 1996-06, Vol.223 (3), p.670-674
Hauptverfasser: Schlyer, Bruce D., Steel, Duncan G., Gafni, Ari
Format: Artikel
Sprache:eng
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Zusammenfassung:Preliminary room temperature phosphorescence measurements of the highly buried Trp109 inE. colialkaline phosphatase have been used to report on the kinetics of protein hydrogen–deuterium exchange. Upon dilution in D2O the phosphorescence lifetime increases (at 20°C) in a biphasic manner with an immediate change (
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0953