Regulation of FGF-4 Enhancer Activity by Transcription Factor NF-Y

Regulation of FGF-4 gene expression is controlled both by elements in the promoter and by an enhancer domain located in the untranslated region of the third exon. We have determined that transcription factor NF-Y binds to the FGF-4 promoter. We further show by mutational analysis that binding of NF-...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1995-06, Vol.211 (2), p.519-527
Hauptverfasser:	Bryans, M., Lucas, J.M., Knobloch, T.J., Wilkie, N.M., Lang, J.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Carcinoma, Embryonal CCAAT-Enhancer-Binding Proteins Cell Line Cloning, Molecular DNA Primers DNA-Binding Proteins - metabolism Enhancer Elements, Genetic Fibroblast Growth Factor 4 Fibroblast Growth Factors - biosynthesis Fibroblast Growth Factors - genetics Gene Expression Regulation, Neoplastic Luciferases - biosynthesis Methylation Mice Molecular Sequence Data Mutagenesis, Site-Directed Plasmids Polymerase Chain Reaction Promoter Regions, Genetic Proto-Oncogene Proteins - biosynthesis Proto-Oncogene Proteins - genetics Recombinant Fusion Proteins - biosynthesis Transcription Factors - metabolism Transfection Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Regulation of FGF-4 gene expression is controlled both by elements in the promoter and by an enhancer domain located in the untranslated region of the third exon. We have determined that transcription factor NF-Y binds to the FGF-4 promoter. We further show by mutational analysis that binding of NF-Y is essential for FGF-4 enhancer activity but has minimal effect on activity of the FGF-4 promoter alone.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1995.1844