Analysis of Ca2+-Binding S100 Proteins in Human Heart by HPLC-Electrospray Mass Spectrometry

Analysis of Ca2+-Binding S100 Proteins in Human Heart by HPLC-Electrospray Mass Spectrometry

Calcium dependent processes are often impaired in myocardial dysfunctions and calcium-binding proteins might be involved as mediators of Ca2+ signals. Here we present a method to assess a footprint of the calcium-binding proteins of the S10O family in human heart. The S100 proteins are purified thro...

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Veröffentlicht in:Biochemical and biophysical research communications 1993-12, Vol.197 (2), p.529-535
Hauptverfasser: Pedrocchi, M., Hauer, C.R., Schafer, B.W., Erne, P., Heizmann, C.W.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Binding and carrier proteins
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Proteins
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Zusammenfassung:Calcium dependent processes are often impaired in myocardial dysfunctions and calcium-binding proteins might be involved as mediators of Ca2+ signals. Here we present a method to assess a footprint of the calcium-binding proteins of the S10O family in human heart. The S100 proteins are purified through calcium dependent affinity chromatography and reverse phase high-performance liquid chromatography and are analyzed by electrospray-ionization mass spectrometry (ESI-MS) and liquid secondary ionization/tandem quadrupole mass spectrometry (LS-MS/MS). In human heart we identified S100α, CACY, and CAPL as the most abundant S100 proteins and showed that they occur as monomers and homodimers.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.2511