Simple and Efficient Method for Isolation and Measurement of Cyanobacterial Hepatotoxins by Plant Tests ( Sinapis alba L.)

A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard ( Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the n...

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Veröffentlicht in:	Analytical biochemistry 1995-02, Vol.225 (1), p.49-53
Hauptverfasser:	Kos, P., Gorzo, G., Suranyi, G., Borbely, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - analysis Bacterial Toxins - isolation & purification Bacterial Toxins - toxicity Biological Assay - methods Chromatography, DEAE-Cellulose - methods Chromatography, Ion Exchange - methods Cyanobacteria - chemistry Mice Microcystins Mustard Plant - drug effects Peptides, Cyclic - analysis Peptides, Cyclic - isolation & purification Peptides, Cyclic - toxicity Plants, Medicinal
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Beschreibung
Zusammenfassung:	A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard ( Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S. alba L. seedling test in the quantitative assessment of the toxin with an IC 50 of 3 μg ml −1 instead of the mouse intraperitoneal test.
ISSN:	0003-2697 1096-0309
DOI:	10.1006/abio.1995.1106