Alterations in the Ah Receptor Level after Staurosporine Treatment

Toxicity of 2,3,7 ,8-tetrachlorodibenzci- p-dioxin (TCDD) is known to be mediated by the aryl hydrocarbon receptor (AhR). The ligand-receptor complex mediates the induction of CYP1A1 (cytochrome P 1450) gene transcription through interaction with an Ah-responsive element. Staurosporine, a potent inhibitor of protein kinases inactivates the TCDD-induced CYPlAl gene transcription. This study was initiated to study binding capacity, capability to translocate, and levels of the AhR in Hepa 1 cells after staurosporine treatment. Levels of the AhR were quantitated in Hepa 1 cells by sandwich type radioimmunochemical method using Rpt 1 (AhR monoclonal antibody) as the primary antibody and [ 125I]goat anti-mouse as the secondary antibody. Staurosporine treatment caused a time- and concentration-dependent decrease in the intracellular concentration of AhR. At 8 h, the EC 50 for the staurosporine-dependent decrease in AhR was 75 nM. Although the receptor levels decreased significantly, it did not affect the properties of the receptor as judged by its ligand binding capacity and functional nuclear translocation of the existing AhR. The iso-electric point of the receptor was essentially unaltered after staurosporine treatment, an indirect indication that the degree of AhR phosphorylation did not change. There could be a variety of explanations for these results such as the decrease in the AhR levels may be due to either an increase in proteolytic activity caused by the imbalance of kinase/phosphatase levels or a decrease in de novo receptor synthesis.