Changes of glycolipids and gangliosides in 2,4-dichlorophenoxyacetic acid treated Chinese hamster ovary cells in monolayer culture

The effects of 2,4‐dichlorophenoxyacetic acid (2,4‐D) on the growth of Chinese hamster ovary cells were studied. 2,4‐D (10−3M) had a cell growth inhibitory effect on CHO cells in monolayer cultures. When 2,4‐D was removed after four days of treatment, a rapid resumption of cell growth rate occurred. At 10−5M 2,4‐D, only a delay of growth was observed, and glycolipid and ganglioside contents were increased in both logarithmic and stationary phases of culture, while no changes in protein, cholesterol, and phospholipids were observed.