A Proposal for a Standardised Protocol to Barcode All Land Plants

We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no...

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Veröffentlicht in:	Taxon 2007-05, Vol.56 (2), p.295-299
Hauptverfasser:	Chase, Mark W., Cowan, Robyn S., Hollingsworth, Peter M., van den Berg, Cassio, Madriñán, Santiago, Petersen, Gitte, Seberg, Ole, Jørgsensen, Tina, Cameron, Kenneth M., Carine, Mark, Pedersen, Niklas, Hedderson, Terry A. J., Conrad, Ferozah, Salazar, Gerardo A., Richardson, James E., Hollingsworth, Michelle L., Barraclough, Timothy G., Kelly, Laura, Wilkinson, Mike
Format:	Artikel
Sprache:	eng
Schlagworte:	Bar codes Botanical gardens Evolution Genetic variation Genomes ITS nrDNA land plant barcoding matK Mitochondrial DNA New Trends in Plant Systematics Plants plastid DNA Plastids Polymerase chain reaction psbA-trnH rbcL rpoB rpoC1 Taxa trnL
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Zusammenfassung:	We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non-coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three-region options, (1) rpoC1, rpoB and matK or (2) rpoC1, matK and psbA-trnH as viable markers for land plant barcoding.
ISSN:	0040-0262 1996-8175
DOI:	10.1002/tax.562004