Front Cover: Simple and rapid analysis of Linagliptin in dried blood spot using an ionic liquid based vortex‐assisted dispersive liquid–liquid microextraction coupled with liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to pharmacokinetic studies

DOI: 10.1002/sscp.202100008 The cover picture shows a simple, rapid and convenient ionic liquid based vortex‐assisted dispersive liquid‐liquid microextraction method for the determination of Linagliptin. The extraction sample was assayed using liquid chromatography tandem‐mass spectrometry using electrospray ionization. The effects of significant factors in extracting and disperser solvent, and salt contents were investigated. MS/MS detection was performed to monitor precursor → product ion transitions m/z 473.3 → 364.2 for Linagliptin, m/z 408.2 → 235.1 for internal standard. The assay was linear from 6–1500 ng/mL and the mean recoveries are more than 90%. The peak dried blood spot concentration (Cmax) after 1.5 h was determined to be 1391.2 ng/mL for Linagliptin.