A Cascade Enzyme System Integrating Peroxidase Mimic with Catalase for Linear Range Expansion of H 2 O 2 Assay: A Mechanism and Application Study
Peroxidase (POD) Nanozyme-based hydrogen peroxide (H O ) detection is popular, but hardly adapt to high concentration of H O owing to narrow linear range (LR) and low LR maximum. Here, a solution of combining POD and catalase (CAT) is raised to expand the LR of H O assay via decomposing part of H O...
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Veröffentlicht in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2023-06, Vol.19 (25), p.e2300444 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Peroxidase (POD) Nanozyme-based hydrogen peroxide (H
O
) detection is popular, but hardly adapt to high concentration of H
O
owing to narrow linear range (LR) and low LR maximum. Here, a solution of combining POD and catalase (CAT) is raised to expand the LR of H
O
assay via decomposing part of H
O
. As a proof of concept, a cascade enzyme system (rGRC) is constructed by integrating ruthenium nanoparticles (RuNPs), CAT and graphene together. The rGRC-based sensor does perform an expanded LR and higher LR maximum for H
O
detection. Meanwhile, it is confirmed that LR expansion is closely associated with apparent K
of rGRC, which is determined by the relative enzyme activity between CAT and POD both in theory and in experiment. At last, rGRC is successfully used to detect high concentration of H
O
(up to 10 mm) in contact lens care solution, which performs higher assay accuracy (close to 100% recovery at 10 mm of H
O
) than traditional POD nanozymes. This study brings up a kind of POD/CAT cascade enzyme system and provides a new concept for accurate and facile H
O
detection. Additionally, it replenishes a new enzyme-substrate model of achieving the same pattern with competitive inhibition in enzyme reactions. |
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ISSN: | 1613-6810 1613-6829 |
DOI: | 10.1002/smll.202300444 |