Graphene‐Gold Hybrid Nanomaterial Based Impedimetric Immunosensor for H3N2 Influenza A Virus Detection

H3N2, known as “swine flu” is a kind of influenza A virus that can also infect humans. Therefore, rapid, practical and accurate techniques for H3N2 detection is needed. In this study, we fabricate Graphene‐gold hybrid nanomaterial modified hemagglutinin‐3 antibody (anti‐H3) based electrochemical immunosensor for practical, sensitive, and selective detection of H3N2 virus. The specific interaction between anti‐H3 and H3 protein on H3N2 virus was monitored with electrochemical impedance spectroscopy. After experimental parameters were optimized, analytical characteristics were searched. A linearity in the range of 1.0–17.5 μg/mL with limit of detection value of 1.01 μg/mL were obtained for the virus. Relative standard deviation value was found as 4.60 % for 15 μg/mL H3N2 virus (n=3). By using synthetic saliva solution that was supplemented with the virus, matrix effect was also investigated. Lastly, the selectivity of developed anti‐H3 based electrochemical influenza A immunosensor was investigated by applying it for H1N1 virus detection. Anti‐H3 antibody based Graphene‐Au hybrid nanomaterial modified immunosensor was developed for the effective, practical and economic detection of H3N2 virus which was selected as model influenza A virus. This developed platform has a potential application to diagnose different types of influenza viruses that contain the H3 protein on their surfaces.