“Turn‐on” Fluorescence Chemosensor Based Probing of Cu 2+ with Excellent Sensitivity: Experimental Study, DFT Calculations and Application in Living Cells and Natural Waters
Developing selective, precise, and rapid determination methods for Cu 2+ sensing is of great significance for biological systems, public health, and environmental protection. Hence, a new coumarin‐based fluorescent chemosensor 3‐((E)‐(((E)‐3,5‐di‐tert‐butyl‐2‐hydroxybenzylidene)hydrazono) methyl)‐7‐...
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Veröffentlicht in: | ChemistrySelect (Weinheim) 2021-06, Vol.6 (24), p.6286-6294 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Developing selective, precise, and rapid determination methods for Cu
2+
sensing is of great significance for biological systems, public health, and environmental protection. Hence, a new coumarin‐based fluorescent chemosensor 3‐((E)‐(((E)‐3,5‐di‐tert‐butyl‐2‐hydroxybenzylidene)hydrazono) methyl)‐7‐(diethylamino)‐2H‐chromen‐2‐one (
HBAC
) for Cu
2+
sensing in MeOH:H
2
O (80 : 20, v:v, pH=7 PBS buffer) media has been constructed.
HBAC
responded to Cu
2+
with high selectivity and exhibited a relatively low LOD of 7.0 nM and ∼22‐fold fluorescent enhancement at 461 nm with the addition of Cu
2+
(0.0‐5.0 μM). The reaction mechanism of the
HBAC
‐Cu
2+
system has been fully examined by MALDI‐TOF‐MS, FT‐IR, and time dependent‐density functional theory (TD‐DFT) calculations. The sensing mechanism was occurred due to the chelation enhanced fluorescence (CHEF) and inhibition of PET phenomena. Furthermore, the chemosensor
HBAC
for Cu
2+
sensing in living systems and natural spring water samples has been successfully utilized, suggesting that the prepared
HBAC
is an appropriate chemosensor for examining Cu
2+
in biological and environmental systems. |
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ISSN: | 2365-6549 2365-6549 |
DOI: | 10.1002/slct.202101060 |