Lipase Immobilized Metal‐Organic Frameworks as Remarkably Biocatalyst for Ester Hydrolysis: A One Step Approach for Lipase Immobilization

Recently, development of smart materials for immobilization of enzyme draws a significant attention in frontier research due to their applications in a wide range of catalytic reactions in several industries. Although numerous supports have already been developed in the past years for enzyme immobilization, challenges still exist to design a material with superior enticing properties like green synthesis approach, high enzyme loading and efficient immobilization process. Here, we have reported an efficient method to immobilize Candida rugosa lipase (CRL) in Zn‐(NH2‐BDC) metal‐organic frameworks (MOFs) via physical adsorption. The main objective of this work is to develop a material for enzyme immobilization that can immobilize enzyme during the synthesis of support. Here, a single step procedure offers both the synthesis of support and enzyme immobilization process. After successful immobilization, it is observed from the systematic characterization that the CRL is loaded 280 mg/g of Zn‐(NH2‐BDC) MOFs during the crystal growth. The immobilized CRL demonstrates promising stability and catalytic activity in the ester hydrolysis. Most importantly, the immobilized CRL retains 79% of its initial activities after 10 times reuse. Our study highlights to develop an elegant material for effective enzyme immobilization. A MOF is developed for enzyme immobilization by one step green synthesis method. The synthesized lipase immobilized MOFs has good loading efficiency (280 mg /g of MOF) and also possess good activity efficiency. The immobilized lipase shows good pH and thermal as well as high storage stability. The immobilized lipase retains 79% of its initial activities after 10 times reuse.