Positive Dendritic Effect on Maleimide Surface Modification of Core‐Shell (γ‐Fe2O3/Polymer) Nanoparticles for Bio‐Immobilization

Maleimide functionalization of γ‐Fe2O3/Polymer MNPs (Magnetic Nanoparticles) was investigated via covalent grafting of a linear and two dendritic functional coupling agents. Chemical surface modification was monitored by ATR FTIR spectroscopy and we emphasized a positive dendritic effect on the surface functionalization. Indeed, the amount of functional group on MNPs’ surface is directly proportional to the dendron's generation of the coupling agent. The immobilization of biomolecules (via thiol/maleimide reaction) was achieved using a two‐steps strategy based on (i) biotinylation of maleimide surfaces and (ii) subsequent specific immobilization of streptavidin coated gold 15 nm NPs (SA−Au). The efficiency of maleimide modified MNPs to react with thiol groups of biomolecules was assessed by UV‐Vis quantification of SA−Au NPs. The selectivity and the specificity of this immobilization was undoubtedly established, and a positive dendritic effect was demonstrated by the immobilization of Streptavidin coated gold nanoparticles on the surface of MNPs. Maleimide functionalization of γ‐Fe2O3/Polymer MNPs was investigated via covalent grafting of a linear and two dendritic functional coupling agents. A positive dendritic effect on the surface modification chemistry was demonstrated. The selective and the specific immobilization of biomolecules was established via thiol/maleimide reaction.