Study by Monte-Carlo simulation of resolution improvement by energy filtering in Bio-TEM

An electron microscope for observing biological samples at atmospheric pressure has been proposed. The major principle of the present instrument is that a biological sample is set in a conventional transmission electron microscope (TEM) beamline but it is isolated from a high vacuum by upper‐ and lower bulkheads. As indicated by an electron trajectory simulation, a clear image could be obtained if the bulkhead was 100 nm thick beryllium foil. In the present study, the feasibility of the image resolution improvement is examined by energy filtering of the transmitted electrons. Copyright © 2002 John Wiley & Sons, Ltd.