Conformational changes and excitation energy transfer in myosin-auramine O system

In the myosin–auramine O system 10 or 11 binding sites of auramine O on the rod‐like part of myosin were discovered. The myosin fluorescence decreases with an increase in auramine O concentration. This is evidence of the excitation energy transfer from tryptophanyls situated near the binding sites to auramine O. The effective distance of energy transfer is 35 Å. It is suggested that the light meromyosin has binding sites with periodicities of 77‐86 Å. Every binding site has a negative charge and hydrophobic locus.