Observation of dislocations in hen egg-white lysozyme crystals by synchrotron monochromatic-beam X-ray topography

Dislocations in large hen egg‐white lysozyme (HEWL) crystals with tetragonal and orthorhombic forms were investigated by means of synchrotron monochromatic‐beam X‐ray topography. For the tetragonal form, not only screw dislocations but also edge ones are found to be included in predominant ones para...

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Veröffentlicht in:	Physica status solidi. A, Applications and materials science Applications and materials science, 2009-08, Vol.206 (8), p.1825-1828
Hauptverfasser:	Mukobayashi, Yu, Kitajima, Natsuko, Yamamoto, Yu, Kajiwara, Kentaro, Sugiyama, Hiroshi, Hirano, Keiichi, Kojima, Kenichi, Tachibana, Masaru
Format:	Artikel
Sprache:	eng
Schlagworte:	61.72.Dd 61.72.Ff Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects, investigation methods Proteins
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Zusammenfassung:	Dislocations in large hen egg‐white lysozyme (HEWL) crystals with tetragonal and orthorhombic forms were investigated by means of synchrotron monochromatic‐beam X‐ray topography. For the tetragonal form, not only screw dislocations but also edge ones are found to be included in predominant ones parallel to 〈110〉 directions. In addition, curved dislocations that can be related to (001)〈110〉 slip system are observed at the crystal edges. For the orthorhombic form, predominant edge dislocations with [001] Burgers vector are stereoscopically observed. It is clarified that they are generated at or around the nucleus centre or sector boundary.
ISSN:	1862-6300 1862-6319
DOI:	10.1002/pssa.200881596