Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol
Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control...
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| Veröffentlicht in: | The Prostate 1986, Vol.8 (3), p.255-264 |
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| container_title | The Prostate |
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| creator | Mobbs, B. G. Johnson, I. E. |
| description | Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control tumors and were not significantly higher in treated tumors: however, in the latter, a significantly higher proportion (73 ± 16%) was associated with the nuclear fraction than in the control tumors ( |
| doi_str_mv | 10.1002/pros.2990080306 |
| format | Article |
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Mean PgR concentrations were more than seven‐fold higher in treated than in control tumors, and approximately 25% of the total was associated with the nuclear fraction in both treated and control tumors, under the assay conditions used. There was a strong linear correlation between nuclear ER and PgR concentrations.
These data indicate that although ER concentration in these tumors was low compared with that in female target organs, it was functional in its ability to associate with the nuclear fraction and induce synthesis of an estrogen‐induced protein (PgR) in proportionate amounts.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.2990080306</identifier><identifier>PMID: 3703745</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Cell Nucleus - analysis ; Cytosol - analysis ; Diethylstilbestrol - pharmacology ; estrogen receptors ; Female ; Male ; progesterone receptors ; Prostate - analysis ; Prostate - drug effects ; Prostatic Neoplasms - chemically induced ; R3327 tumor ; Rats ; Receptors, Estrogen - analysis ; Receptors, Estrogen - drug effects ; Receptors, Progesterone - analysis ; Receptors, Progesterone - drug effects</subject><ispartof>The Prostate, 1986, Vol.8 (3), p.255-264</ispartof><rights>Copyright © 1986 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3826-8a62a4cc13eecc40008d3752d2ea252217d712435f141c157e454a12de4ee76a3</citedby><cites>FETCH-LOGICAL-c3826-8a62a4cc13eecc40008d3752d2ea252217d712435f141c157e454a12de4ee76a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.2990080306$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.2990080306$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3703745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mobbs, B. G.</creatorcontrib><creatorcontrib>Johnson, I. E.</creatorcontrib><title>Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control tumors and were not significantly higher in treated tumors: however, in the latter, a significantly higher proportion (73 ± 16%) was associated with the nuclear fraction than in the control tumors (<33%).
Mean PgR concentrations were more than seven‐fold higher in treated than in control tumors, and approximately 25% of the total was associated with the nuclear fraction in both treated and control tumors, under the assay conditions used. There was a strong linear correlation between nuclear ER and PgR concentrations.
These data indicate that although ER concentration in these tumors was low compared with that in female target organs, it was functional in its ability to associate with the nuclear fraction and induce synthesis of an estrogen‐induced protein (PgR) in proportionate amounts.</description><subject>Animals</subject><subject>Cell Nucleus - analysis</subject><subject>Cytosol - analysis</subject><subject>Diethylstilbestrol - pharmacology</subject><subject>estrogen receptors</subject><subject>Female</subject><subject>Male</subject><subject>progesterone receptors</subject><subject>Prostate - analysis</subject><subject>Prostate - drug effects</subject><subject>Prostatic Neoplasms - chemically induced</subject><subject>R3327 tumor</subject><subject>Rats</subject><subject>Receptors, Estrogen - analysis</subject><subject>Receptors, Estrogen - drug effects</subject><subject>Receptors, Progesterone - analysis</subject><subject>Receptors, Progesterone - drug effects</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUE1PGzEQtapWENKee0LyH9gw_th1Ik4VKhQpgpBSIfViOd5JY9isV7bTkN_DH8XbIFBPnGY0b96beY-QrwxGDICfdMHHEZ9MAMYgoPpABgwmqgCQ5UcyAK6gkEyoQ3IU4z1A5gA_IAdCgVCyHJCnm41pk0smub9IAza58W1cuS7SBaYtYkvtLvnoG2ramrYb26AJFGMK_k8G-2HXtzFh8G2vYbFLPkTqWppWSOdCcNXvxP6KpdYE61q_NtQvaTAp0hTQJKzp1qUVrR2m1a6JyTWLf1eaz-TT0jQRv7zUIfl1_v327Ecxvb64PPs2LawY86oYm4obaS0TiNbK7HRcC1XymqPhJedM1YpxKcolk8yyUqEspWG8RomoKiOG5GSva_OvMeBSd8GtTdhpBrpPW_cm9FvamXG8Z3SbxRrr1_2XeDN-use3rsHde3J6Nr_--Z96sWe7nO3jK9uEB12p7EzfXV3oajqb_i7vZvpWPAOwQaDw</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Mobbs, B. G.</creator><creator>Johnson, I. E.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1986</creationdate><title>Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol</title><author>Mobbs, B. G. ; Johnson, I. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3826-8a62a4cc13eecc40008d3752d2ea252217d712435f141c157e454a12de4ee76a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Cell Nucleus - analysis</topic><topic>Cytosol - analysis</topic><topic>Diethylstilbestrol - pharmacology</topic><topic>estrogen receptors</topic><topic>Female</topic><topic>Male</topic><topic>progesterone receptors</topic><topic>Prostate - analysis</topic><topic>Prostate - drug effects</topic><topic>Prostatic Neoplasms - chemically induced</topic><topic>R3327 tumor</topic><topic>Rats</topic><topic>Receptors, Estrogen - analysis</topic><topic>Receptors, Estrogen - drug effects</topic><topic>Receptors, Progesterone - analysis</topic><topic>Receptors, Progesterone - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mobbs, B. G.</creatorcontrib><creatorcontrib>Johnson, I. E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mobbs, B. G.</au><au>Johnson, I. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>1986</date><risdate>1986</risdate><volume>8</volume><issue>3</issue><spage>255</spage><epage>264</epage><pages>255-264</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control tumors and were not significantly higher in treated tumors: however, in the latter, a significantly higher proportion (73 ± 16%) was associated with the nuclear fraction than in the control tumors (<33%).
Mean PgR concentrations were more than seven‐fold higher in treated than in control tumors, and approximately 25% of the total was associated with the nuclear fraction in both treated and control tumors, under the assay conditions used. There was a strong linear correlation between nuclear ER and PgR concentrations.
These data indicate that although ER concentration in these tumors was low compared with that in female target organs, it was functional in its ability to associate with the nuclear fraction and induce synthesis of an estrogen‐induced protein (PgR) in proportionate amounts.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3703745</pmid><doi>10.1002/pros.2990080306</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0270-4137 |
| ispartof | The Prostate, 1986, Vol.8 (3), p.255-264 |
| issn | 0270-4137 1097-0045 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_pros_2990080306 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell Nucleus - analysis Cytosol - analysis Diethylstilbestrol - pharmacology estrogen receptors Female Male progesterone receptors Prostate - analysis Prostate - drug effects Prostatic Neoplasms - chemically induced R3327 tumor Rats Receptors, Estrogen - analysis Receptors, Estrogen - drug effects Receptors, Progesterone - analysis Receptors, Progesterone - drug effects |
| title | Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol |
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