Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol

Quantitative relationships between cytosol and nuclear estrogen and progesterone receptors in the R3327 prostatic carcinoma of rats treated with diethylstilbestrol

Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control...

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Veröffentlicht in:The Prostate 1986, Vol.8 (3), p.255-264
Hauptverfasser: Mobbs, B. G., Johnson, I. E.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Nucleus - analysis
Cytosol - analysis
Diethylstilbestrol - pharmacology
estrogen receptors
Female
Male
progesterone receptors
Prostate - analysis
Prostate - drug effects
Prostatic Neoplasms - chemically induced
R3327 tumor
Rats
Receptors, Estrogen - analysis
Receptors, Estrogen - drug effects
Receptors, Progesterone - analysis
Receptors, Progesterone - drug effects
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Zusammenfassung:Estrogen and progesterone receptor (ER, PgR) were quantitated by Scatchard analysis in cytosolic and nuclear extracts of R3327H prostatic carcinomas from rats injected with 100 μg diethylstilbestrol or with vehicle for 1–3 weeks. Total tissue ER concentrations were less than 80 fm/mg DNA in control tumors and were not significantly higher in treated tumors: however, in the latter, a significantly higher proportion (73 ± 16%) was associated with the nuclear fraction than in the control tumors (
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.2990080306