Human laminin-5 and laminin-10 mediated gene expression of prostate carcinoma cells

In prostate cancer progression, the basal lamina switches from predominantly laminin‐5 to laminin‐10. DU‐145 prostate cancer cells were treated with either soluble laminin‐5 (20 ng/ml) or laminin‐10 (1 µg/ml) for 6, 24, and 48 hr. Total RNA was harvested for a 7,500 human cDNA microarray. Hybridizations were carried out in accordance with a 10 sample analysis of variance (ANOVA) statistical model. One thousand one hundred sixteen genes had measurable expression 2 standard deviations above background and 50% of spots for any given sample for all hybridizations were positive. Expression values of significantly varying genes were clustered and a list of 408 genes (P