Ammonia Plasma Functionalized Polycarbonate Surfaces Improve Cell Migration Inside an Artificial 3D Cell Culture Module

A three‐dimensional (3D) cell culture module was used to test the requirements of scaffold material properties for improved bone cell ingrowth. For this purpose polycarbonate (PC) samples were treated with microwave‐excited ammonia plasma in a defined time frame of 1–300 s and the influence on initial attachment of osteoblast‐like cells was determined. Perforated PC slices were treated by 30 s of ammonia plasma and mounted on a clamp for 3D cell ingrowth. Plasma treatment conditions were ascertained to stimulate cell adhesion and the migration of human bone cells into the depth of this artificial scaffold. The improvement of cell growth inside of 3D scaffolds is a challenging subject in implantology. A three dimensional cell culture module is constructed to investigate bone cell migration inside an artificial scaffold. Surface properties of the polycarbonate scaffold are optimized by ammonia plasma. This cell culture module is trendsetting for large volume tissue replacement.