Quantitative determination of podophyllotoxin and related compounds in podophyllum species by reverse phase high performance liquid chromatography

A rapid sensitive and reliable reverse phase high performance liquid chromatography method was developed using a Taxsilr̀ column and gradient elution for the routine determination of podophyllotoxin and six related compounds, epipodophyllotoxin, α‐peltatin, β‐peltatin, 4′‐demethylpodophyllotoxin, podophyllotoxin‐4‐O‐glycoside, and 1,2,3,4‐dehydrodesoxypodophyllotoxin in Podophyllum extracts. The elaborated method gave well resolved peaks for these compounds with good detection response and linearity in the range 25–450 ng.