Standardization of the indian crude drug kalmegh by high pressure liquid chromatographic determination of andrographolide

The popular hepatoprotective Indian herbal drug Kalmegh (Andrographis paniculata) can be standardized by high pressure liquid chromatographic determination of its major active constituent, andrographolide. The leaves of the herb were found to contain the highest amount (2.39%w/w) of andrographolide and the seeds to contain the lowest. The technique was found to be much more sensitive and simple than the known spectrophotometric method.