Separation and identification of flavone, flavonol, isoflavone and flavanone aglycones by capillary column gas chromatography

A rapid and sensitive capillary column gas chromatographic method for the separation and identification of trimethylsilyl derivatives of flavonoid aglycones is reported. Well‐resolved, sharp and symmetrical peaks were obtained for a wide range of flavone, flavonol, isoflavone and flavanone aglycones with a variety of substitution patterns. Chromatographic behaviour and the effects of the number, position and type of the substituents on the retention times of these compounds have been defined.