Monodisperse polymer-based stationary phase for carbohydrate analysis by reduced-diameter column anion-exchange chromatography with pulsed amperometric detector

A monodisperse polymer‐based anion exchanger was synthesized through a two‐step swelling and polymerization method for the analysis of unmodified carbohydrates by reduced‐diameter column liquid chromatography with a pulsed amperometric detector. Separation characteristics were studied for several di...

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Veröffentlicht in:The journal of microcolumn separations 2001, Vol.13 (1), p.8-12
Hauptverfasser: Ohkubo, Aya, Shirota, Osamu, Kobayashi, Atsushi, Kimura, Tomohiko, Ohtsu, Yutaka, Hosoya, Ken, Tanaka, Nobuo
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Sprache:eng
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Zusammenfassung:A monodisperse polymer‐based anion exchanger was synthesized through a two‐step swelling and polymerization method for the analysis of unmodified carbohydrates by reduced‐diameter column liquid chromatography with a pulsed amperometric detector. Separation characteristics were studied for several different classes of carbohydrates, including sugar phosphates. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 8–12, 2001
ISSN:1040-7685
1520-667X
DOI:10.1002/mcs.1012