Studies of formation of bivalent metal ion complexes with poly-U
Differential UV spectroscopy is employed to investigate the interaction of poly‐U with Mg2+, Ca2+, Mn2+ and Cu2+ ions in aqueous solution. Mg2+ and Mn2+ ions are observed to bind only to the polynucleotide phosphates, while Cu2+ and Ca2+ also interact with the poly‐U bases. The most probable coordin...
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Veröffentlicht in: | Die Makromolekulare Chemie 1989-07, Vol.190 (7), p.1595-1602 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Differential UV spectroscopy is employed to investigate the interaction of poly‐U with Mg2+, Ca2+, Mn2+ and Cu2+ ions in aqueous solution. Mg2+ and Mn2+ ions are observed to bind only to the polynucleotide phosphates, while Cu2+ and Ca2+ also interact with the poly‐U bases. The most probable coordination sites are O(4) and also N(3) for Cu2+ ions. N(3) is a coordination site of Cu2+ ions in UMP and uridine. The rest of the ions do not interact with the bases of the free monomers. Unlike the non‐cooperative Cu2+‐UMP binding, the ion interaction (including Ca2+) with the poly‐U bases is characterized by a positive cooperativity. The association constant of Ca2+ (45 1.mol−1) is 45 times lower than that of Cu2+. |
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ISSN: | 0025-116X 0025-116X |
DOI: | 10.1002/macp.1989.021900711 |