Studies of formation of bivalent metal ion complexes with poly-U

Differential UV spectroscopy is employed to investigate the interaction of poly‐U with Mg2+, Ca2+, Mn2+ and Cu2+ ions in aqueous solution. Mg2+ and Mn2+ ions are observed to bind only to the polynucleotide phosphates, while Cu2+ and Ca2+ also interact with the poly‐U bases. The most probable coordin...

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Veröffentlicht in:Die Makromolekulare Chemie 1989-07, Vol.190 (7), p.1595-1602
Hauptverfasser: Sorokin, Victor A., Levchenko, Irina V., Valeev, Vladimir A., Gladchenko, Galina O., Blagoi, Yuri P., Li, Ren G.
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Sprache:eng
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Zusammenfassung:Differential UV spectroscopy is employed to investigate the interaction of poly‐U with Mg2+, Ca2+, Mn2+ and Cu2+ ions in aqueous solution. Mg2+ and Mn2+ ions are observed to bind only to the polynucleotide phosphates, while Cu2+ and Ca2+ also interact with the poly‐U bases. The most probable coordination sites are O(4) and also N(3) for Cu2+ ions. N(3) is a coordination site of Cu2+ ions in UMP and uridine. The rest of the ions do not interact with the bases of the free monomers. Unlike the non‐cooperative Cu2+‐UMP binding, the ion interaction (including Ca2+) with the poly‐U bases is characterized by a positive cooperativity. The association constant of Ca2+ (45 1.mol−1) is 45 times lower than that of Cu2+.
ISSN:0025-116X
0025-116X
DOI:10.1002/macp.1989.021900711