Effect of the Sialylation State of α1-Acid Glycoprotein on Propranolol Binding

α1-Acid glycoprotein (AAG) exists in a variety of sialylated states which can be influenced by certain disease states. The purpose of the present study was to establish the effect of the sialylation state of AAG on the binding of a model cationic drug (propranolol). Extensive in vitro enzymatic desi...

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Veröffentlicht in:Journal of pharmaceutical sciences 1985-04, Vol.74 (4), p.473-475
Hauptverfasser: Primozic, S., McNamara, P.J.
Format: Artikel
Sprache:eng
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Zusammenfassung:α1-Acid glycoprotein (AAG) exists in a variety of sialylated states which can be influenced by certain disease states. The purpose of the present study was to establish the effect of the sialylation state of AAG on the binding of a model cationic drug (propranolol). Extensive in vitro enzymatic desialylation of isolated human AAG was achieved utilizing neuraminidase bonded to agarose beads. Propranolol binding to native and desialylated AAG was determined by equilibrium dialysis. Desialylated AAG exhibited a modest increase in propranolol free fraction due to a decrease in the affinity constant (Ka) compared to untreated AAG (3.3 × 105 M−1 versus 4.0 × 105 M−1, respectively). A modest 15% increase was predicted for the free fraction of propranolol in serum containing desialylated AAG at therapeutic propranolol concentrations (
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600740421