Biophysical Characterization of PEI/DNA Complexes

The main goal of this study was to determine the effects of polyethylenimine (PEI) molecular weight and structure (750kDa, 25kDa, 2kDa branched, and 25kDa linear PEI) and the nitrogen/phosphate (N/P) molar ratio on the physical properties and transfection efficiencies of PEI/DNA complexes. Fourier t...

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Veröffentlicht in:	Journal of pharmaceutical sciences 2003-08, Vol.92 (8), p.1710-1722
Hauptverfasser:	Choosakoonkriang, Sirirat, Lobo, Brian A., Koe, Gary S., Koe, Janet G., Middaugh, C.Russell
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biophysical Phenomena Biophysics Cercopithecus aethiops CHO Cells circular dichroism COS Cells Cricetinae differential scanning calorimetry DNA - analysis DNA - chemistry DNA - genetics DNA delivery FTIR gene delivery General pharmacology Genetic Therapy - methods isothermal titration calorimetry Medical sciences Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments physical characterization plasmid DNA Polyethyleneimine - analysis Polyethyleneimine - chemistry polyethylenimine Spectroscopy, Fourier Transform Infrared - methods Transfection - methods
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Zusammenfassung:	The main goal of this study was to determine the effects of polyethylenimine (PEI) molecular weight and structure (750kDa, 25kDa, 2kDa branched, and 25kDa linear PEI) and the nitrogen/phosphate (N/P) molar ratio on the physical properties and transfection efficiencies of PEI/DNA complexes. Fourier transform infrared spectroscopy revealed that DNA remained in the B conformation when complexed to all PEIs. Unique alterations in the circular dichroism spectra of DNA were observed in the presence of each PEI, whereas differential scanning calorimetry measurements showed that all PEIs examined destabilized supercoiled DNA at N/P
ISSN:	0022-3549 1520-6017
DOI:	10.1002/jps.10437