Metabolism of aniline and 3-chloroaniline in Pseudomonas acidovorans CA28: Evidence of isofunctional muconate cycloisomerases

Two distinct muconate cycloisomerases are involved in the degradation of aniline and 3‐chloroaniline (3‐CA) proceeding via the ortho‐cleavage pathways in Pseudomonas acidovorans CA28. After partial purification of these two enzymes kinetic investigations resulted in a clear differentiation of aniline‐derived muconate cycloisomerase (MC) and 3‐CA‐derived chloromuconate cycloisomerase (CMC). A further result of this study revealed the simultaneous coexistence of MC and CMC in strain CA28 when grown on a mixture of aniline and 3‐CA.