Auswirkungen von Mutanteninduktion und Protoplastenfusion auf ausgewählte Dehydrogenase‐Muster von Pichia guilliermondii

We investigated the enzyme patterns of two wild‐type strains, two auxotrophic mutants and some fusion hybrids of Pichia guilliermondii after the electrophoretic separation of their crude cell extracts in dependence on the cultivation on mineral salt medium with different carbon sources. The wild‐type strains showed a constitutive synthesis of one NAD‐specific and some NADP‐specific enzymes and their ability to oxidize primary alcohols with a chain length from C2 to C8. After the induction of mutants auxotroph for amino acids, nicotinic acid or adenine we found different enzyme patterns, too. Prototrophic fusion hybrids obtained via protoplast fusion of the two double auxotrophic mutants showed behaviour either characteristic for the wild‐type strains or characteristics of their parental strains and in some cases also combination of parental enzyme patterns. The behaviour of the hybrids seems to be dependent on the quantity of DNA transferred into each fusion hybrid from the parental mutant strains. So the method of protoplast fusion allows to get qualitatively different hybrids.