Tandem mass specatrometry of carbonic anhydrase (29 kDa)

Tandem mass specatrometry of carbonic anhydrase (29 kDa)

Using electrospray ionization with a Fourier‐transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings. Such sensitive and specific direct characterization of proteins ap...

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Veröffentlicht in:Journal of mass spectrometry. 1995-01, Vol.30 (1), p.88-93
Hauptverfasser: O'Connor, Peter B., Speir, J. Paul, Senko, Michael W., Little, Daniel P., McLafferty, Fred W.
Format: Artikel
Sprache:eng
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Zusammenfassung:Using electrospray ionization with a Fourier‐transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings. Such sensitive and specific direct characterization of proteins appears to be complementary to conventional techniques including LC/MS/MS sequencing of oligopeptides from protein degradation.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.1190300114