Preparation of [14C]Acetamidino-insulin

Preparation of [14C]Acetamidino-insulin

[14C]Acetamidino‐insulin was synthesized by acetamidination of insulin with methyl [14C]acetimidate hydrochloride. The acetamidino‐insulin contains two main components, which can be separated by DEAE‐cellulose column chromatography. They correspond to glycineA‐1 ‐ PhenylalanineB‐1 ‐ Nε ‐ lysineB‐29...

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Veröffentlicht in:Journal of labelled compounds & radiopharmaceuticals 1980-09, Vol.17 (5), p.699-706
Hauptverfasser: Matsuo, Mitsuyoshi, Yamauchi, Hirose, Ooyama, Toshiro, Orimo, Hajime
Format: Artikel
Sprache:eng
Schlagworte:
Acetamidination
Carbon-14
DEAE-cellulose column chromatography
Insulin
Methyl acetimidate
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Beschreibung
Zusammenfassung:[14C]Acetamidino‐insulin was synthesized by acetamidination of insulin with methyl [14C]acetimidate hydrochloride. The acetamidino‐insulin contains two main components, which can be separated by DEAE‐cellulose column chromatography. They correspond to glycineA‐1 ‐ PhenylalanineB‐1 ‐ Nε ‐ lysineB‐29 ‐ [14C]acetamidino‐insulin and glycineA‐1 ‐ Nε ‐ lysineB‐29 ‐ [14C]acetamidino‐insulin. These immunological and biological activity was compared with that of native insulin.
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.2580170512