Biosynthesis of tritium, deuterium and carbon-13 labeled cycloheximide

Biosynthesis of tritium, deuterium and carbon-13 labeled cycloheximide

Tritium, deuterium, and carbon‐13 labeled cycloheximide were prepared through fermentation of Streptomyces griseus in the presence of S‐methyl labeled L‐methionine. Cycloheximide‐3H of specific activity 4.96 mCi/mg (1.40 Ci/mmol) was obtained in 25% overall radiochemical yield. Nuclear magnetic reso...

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Veröffentlicht in:Journal of labelled compounds & radiopharmaceuticals 1980-09, Vol.17 (5), p.613-626
Hauptverfasser: Hsi, Richard S. P., Witz, Dennis F., Stolle, Wayne T., Baczynskyj, Lubomir, Scahill, Terrence A.
Format: Artikel
Sprache:eng
Schlagworte:
biosynthesis
carbon-13
Cycloheximide
deuterium
tritium
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Zusammenfassung:Tritium, deuterium, and carbon‐13 labeled cycloheximide were prepared through fermentation of Streptomyces griseus in the presence of S‐methyl labeled L‐methionine. Cycloheximide‐3H of specific activity 4.96 mCi/mg (1.40 Ci/mmol) was obtained in 25% overall radiochemical yield. Nuclear magnetic resonance and mass spectral analyses of the deuterium and carbon‐13 labeled products showed that isotope incorporation occurred virtually exclusively in the two methyl groups in cycloheximide, with negligible (
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.2580170503