Novel and efficient detection of cAMP-dependent protein kinase for the development of a protein biochip

Protein microarrays have recently become a powerful research tool for proteomics and clinical investigations. A novel and efficient detection of cAMP‐dependent protein kinase for the development of a protein biochip has been established. The phosphorylation of a substrate by [γ‐33P]ATP and [γ‐32P]ATP was performed on a glass chip, and a facile detection of a kinase assay using 32P and 33P on a glass chip was accomplished by a radio‐TLC imaging scanner. Since the radio‐TLC detection method takes shorter detection time (2 min) than those required for X‐ray film or bioimage analyzer (6–12 h), the latter can be replaced. The detection by the radio‐TLC was proven to be rapid and suitable for a kinase assay using radioisotopes for developing a biochip. Copyright © 2009 John Wiley & Sons, Ltd. Radio‐TLC detection for a phosphorylation assay using RI's on a microarray has been established. Since the radio‐TLC detection method takes shorter detection time (2 min) than those required for x‐ray film or bioimage analyzer (6‐12 hr), the latter can be replaced. Copyright © 2009 John Wiley & Sons, Ltd.