High-performance liquid chromatography of proteins on short capillary columns

A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by the miniaturiz...

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Veröffentlicht in:	Journal of high resolution chromatography 1990-03, Vol.13 (3), p.185-189
Hauptverfasser:	Mal'tsev, V. G., Nasledov, D. G., Trushin, S. A., Tennikova, T. B., Vinogradova, L. V., Volokitina, I. N., Zgonnik, V. N., Belenkii, Boris G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects, investigation methods Micro-LC Miniaturization Protein analysis Proteins Short capillaries
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by the miniaturization of the column and the optimization of gradient steepness.
ISSN:	0935-6304 1521-4168
DOI:	10.1002/jhrc.1240130310