Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors
The C (RH:8), C (RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C and C are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C (RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Fin...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2018-10, Vol.32 (8), p.e22570 |
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| creator | Costa, Sidneia S Souza Silva, Thamy C Chiba, Akemi Kuroda Cruz, Bruno R Langhi Junior, Dante Mário Bordin, José O |
| description | The C
(RH:8), C
(RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C
and C
are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C
(RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Finns, and 9% in Latvians. The aim of this study was to determine the frequency of RhD+ phenotypes in a population of southeast Brazilian blood donors and to perform a molecular study to distinguish the RHCE*Ce.08.01 and RHCE*Ce.09 alleles, responsible for the C
and C
expressions, respectively.
We investigated 11,536 RhD+ Brazilian blood donors. All samples were phenotyped for D, C, c, E, e, and C
. In the C
+ samples, a molecular analysis was performed to detect the nucleotide substitutions A122G and G106A, which determine the C
and C
antigens, respectively.
C
antigen was found in 110 (0.95%) samples in the following phenotypes: DC
e/dC
e (72/0.62%), DC
e/DC
e (30/0.26%), and DC
e/DC
E (8/0.07%). Among 110 C
+ samples, 108 showed the A122G nucleotide substitution associated with RHCE*Ce.08.01 allele and 2 samples the G106A substitution associated with the RHCE*Ce.09.01 allele.
This study showed the prevalence of the RhD+ phenotype in the Brazilian population, and that through the molecular study, it was possible to differentiate the RHCE*Ce.08.01 and RHCE*Ce.09.01 alleles. The phenotype frequency was similar from Black people (1%) and different from Caucasians, Finns, and Latvians. |
| doi_str_mv | 10.1002/jcla.22570 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_jcla_22570</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>29926983</sourcerecordid><originalsourceid>FETCH-LOGICAL-c143t-b7a269bc8b8dd607831b86f35d1675edd973c32d6998adcdee37d43badff93753</originalsourceid><addsrcrecordid>eNo9kF1LwzAYhYMobk5v_AGSa6FbPtYmudTiF0wE0euS5k22jq6pSYvWX-8-1KtzLh4OhwehS0qmlBA2W5taTxlLBTlCY0qUTJhk6TEaEylFIgnlI3QW45oQIhXNTtGIKcUyJfkYLZ99bU1f64Bj18OAvcM5_sSzHH9h3XTV0jZxWwC7YD9625g98rrC7co2vhtaG3HV4Oj7bmV17PBt0N9VXekGl7X3gME3PsRzdOJ0He3Fb07Q-_3dW_6YLF4envKbRWLonHdJKfT2WGlkKQEyIiSnpcwcT4FmIrUASnDDGWRKSQ0GrOUC5rzU4JziIuUTdH3YNcHHGKwr2lBtdBgKSoqdrWJnq9jb2sJXB7jty42Ff_RPD_8Bphxm_Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Costa, Sidneia S ; Souza Silva, Thamy C ; Chiba, Akemi Kuroda ; Cruz, Bruno R ; Langhi Junior, Dante Mário ; Bordin, José O</creator><creatorcontrib>Costa, Sidneia S ; Souza Silva, Thamy C ; Chiba, Akemi Kuroda ; Cruz, Bruno R ; Langhi Junior, Dante Mário ; Bordin, José O</creatorcontrib><description>The C
(RH:8), C
(RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C
and C
are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C
(RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Finns, and 9% in Latvians. The aim of this study was to determine the frequency of RhD+ phenotypes in a population of southeast Brazilian blood donors and to perform a molecular study to distinguish the RHCE*Ce.08.01 and RHCE*Ce.09 alleles, responsible for the C
and C
expressions, respectively.
We investigated 11,536 RhD+ Brazilian blood donors. All samples were phenotyped for D, C, c, E, e, and C
. In the C
+ samples, a molecular analysis was performed to detect the nucleotide substitutions A122G and G106A, which determine the C
and C
antigens, respectively.
C
antigen was found in 110 (0.95%) samples in the following phenotypes: DC
e/dC
e (72/0.62%), DC
e/DC
e (30/0.26%), and DC
e/DC
E (8/0.07%). Among 110 C
+ samples, 108 showed the A122G nucleotide substitution associated with RHCE*Ce.08.01 allele and 2 samples the G106A substitution associated with the RHCE*Ce.09.01 allele.
This study showed the prevalence of the RhD+ phenotype in the Brazilian population, and that through the molecular study, it was possible to differentiate the RHCE*Ce.08.01 and RHCE*Ce.09.01 alleles. The phenotype frequency was similar from Black people (1%) and different from Caucasians, Finns, and Latvians.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.22570</identifier><identifier>PMID: 29926983</identifier><language>eng</language><publisher>United States</publisher><subject>Alleles ; Blood Donors - statistics & numerical data ; Brazil ; Cross-Sectional Studies ; Gene Frequency ; Genotyping Techniques ; Humans ; Rh-Hr Blood-Group System - genetics</subject><ispartof>Journal of clinical laboratory analysis, 2018-10, Vol.32 (8), p.e22570</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c143t-b7a269bc8b8dd607831b86f35d1675edd973c32d6998adcdee37d43badff93753</cites><orcidid>0000-0001-6684-890X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29926983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Costa, Sidneia S</creatorcontrib><creatorcontrib>Souza Silva, Thamy C</creatorcontrib><creatorcontrib>Chiba, Akemi Kuroda</creatorcontrib><creatorcontrib>Cruz, Bruno R</creatorcontrib><creatorcontrib>Langhi Junior, Dante Mário</creatorcontrib><creatorcontrib>Bordin, José O</creatorcontrib><title>Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors</title><title>Journal of clinical laboratory analysis</title><addtitle>J Clin Lab Anal</addtitle><description>The C
(RH:8), C
(RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C
and C
are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C
(RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Finns, and 9% in Latvians. The aim of this study was to determine the frequency of RhD+ phenotypes in a population of southeast Brazilian blood donors and to perform a molecular study to distinguish the RHCE*Ce.08.01 and RHCE*Ce.09 alleles, responsible for the C
and C
expressions, respectively.
We investigated 11,536 RhD+ Brazilian blood donors. All samples were phenotyped for D, C, c, E, e, and C
. In the C
+ samples, a molecular analysis was performed to detect the nucleotide substitutions A122G and G106A, which determine the C
and C
antigens, respectively.
C
antigen was found in 110 (0.95%) samples in the following phenotypes: DC
e/dC
e (72/0.62%), DC
e/DC
e (30/0.26%), and DC
e/DC
E (8/0.07%). Among 110 C
+ samples, 108 showed the A122G nucleotide substitution associated with RHCE*Ce.08.01 allele and 2 samples the G106A substitution associated with the RHCE*Ce.09.01 allele.
This study showed the prevalence of the RhD+ phenotype in the Brazilian population, and that through the molecular study, it was possible to differentiate the RHCE*Ce.08.01 and RHCE*Ce.09.01 alleles. The phenotype frequency was similar from Black people (1%) and different from Caucasians, Finns, and Latvians.</description><subject>Alleles</subject><subject>Blood Donors - statistics & numerical data</subject><subject>Brazil</subject><subject>Cross-Sectional Studies</subject><subject>Gene Frequency</subject><subject>Genotyping Techniques</subject><subject>Humans</subject><subject>Rh-Hr Blood-Group System - genetics</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LwzAYhYMobk5v_AGSa6FbPtYmudTiF0wE0euS5k22jq6pSYvWX-8-1KtzLh4OhwehS0qmlBA2W5taTxlLBTlCY0qUTJhk6TEaEylFIgnlI3QW45oQIhXNTtGIKcUyJfkYLZ99bU1f64Bj18OAvcM5_sSzHH9h3XTV0jZxWwC7YD9625g98rrC7co2vhtaG3HV4Oj7bmV17PBt0N9VXekGl7X3gME3PsRzdOJ0He3Fb07Q-_3dW_6YLF4envKbRWLonHdJKfT2WGlkKQEyIiSnpcwcT4FmIrUASnDDGWRKSQ0GrOUC5rzU4JziIuUTdH3YNcHHGKwr2lBtdBgKSoqdrWJnq9jb2sJXB7jty42Ff_RPD_8Bphxm_Q</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Costa, Sidneia S</creator><creator>Souza Silva, Thamy C</creator><creator>Chiba, Akemi Kuroda</creator><creator>Cruz, Bruno R</creator><creator>Langhi Junior, Dante Mário</creator><creator>Bordin, José O</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-6684-890X</orcidid></search><sort><creationdate>201810</creationdate><title>Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors</title><author>Costa, Sidneia S ; Souza Silva, Thamy C ; Chiba, Akemi Kuroda ; Cruz, Bruno R ; Langhi Junior, Dante Mário ; Bordin, José O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c143t-b7a269bc8b8dd607831b86f35d1675edd973c32d6998adcdee37d43badff93753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alleles</topic><topic>Blood Donors - statistics & numerical data</topic><topic>Brazil</topic><topic>Cross-Sectional Studies</topic><topic>Gene Frequency</topic><topic>Genotyping Techniques</topic><topic>Humans</topic><topic>Rh-Hr Blood-Group System - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Costa, Sidneia S</creatorcontrib><creatorcontrib>Souza Silva, Thamy C</creatorcontrib><creatorcontrib>Chiba, Akemi Kuroda</creatorcontrib><creatorcontrib>Cruz, Bruno R</creatorcontrib><creatorcontrib>Langhi Junior, Dante Mário</creatorcontrib><creatorcontrib>Bordin, José O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Costa, Sidneia S</au><au>Souza Silva, Thamy C</au><au>Chiba, Akemi Kuroda</au><au>Cruz, Bruno R</au><au>Langhi Junior, Dante Mário</au><au>Bordin, José O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J Clin Lab Anal</addtitle><date>2018-10</date><risdate>2018</risdate><volume>32</volume><issue>8</issue><spage>e22570</spage><pages>e22570-</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>The C
(RH:8), C
(RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C
and C
are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C
(RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Finns, and 9% in Latvians. The aim of this study was to determine the frequency of RhD+ phenotypes in a population of southeast Brazilian blood donors and to perform a molecular study to distinguish the RHCE*Ce.08.01 and RHCE*Ce.09 alleles, responsible for the C
and C
expressions, respectively.
We investigated 11,536 RhD+ Brazilian blood donors. All samples were phenotyped for D, C, c, E, e, and C
. In the C
+ samples, a molecular analysis was performed to detect the nucleotide substitutions A122G and G106A, which determine the C
and C
antigens, respectively.
C
antigen was found in 110 (0.95%) samples in the following phenotypes: DC
e/dC
e (72/0.62%), DC
e/DC
e (30/0.26%), and DC
e/DC
E (8/0.07%). Among 110 C
+ samples, 108 showed the A122G nucleotide substitution associated with RHCE*Ce.08.01 allele and 2 samples the G106A substitution associated with the RHCE*Ce.09.01 allele.
This study showed the prevalence of the RhD+ phenotype in the Brazilian population, and that through the molecular study, it was possible to differentiate the RHCE*Ce.08.01 and RHCE*Ce.09.01 alleles. The phenotype frequency was similar from Black people (1%) and different from Caucasians, Finns, and Latvians.</abstract><cop>United States</cop><pmid>29926983</pmid><doi>10.1002/jcla.22570</doi><orcidid>https://orcid.org/0000-0001-6684-890X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of clinical laboratory analysis, 2018-10, Vol.32 (8), p.e22570 |
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| language | eng |
| recordid | cdi_crossref_primary_10_1002_jcla_22570 |
| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Alleles Blood Donors - statistics & numerical data Brazil Cross-Sectional Studies Gene Frequency Genotyping Techniques Humans Rh-Hr Blood-Group System - genetics |
| title | Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors |
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