Molecular study of C w /C x antigens and frequency of Rh phenotypes in southeast Brazilian blood donors
The C (RH:8), C (RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C and C are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C (RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Fin...
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Veröffentlicht in: | Journal of clinical laboratory analysis 2018-10, Vol.32 (8), p.e22570 |
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Zusammenfassung: | The C
(RH:8), C
(RH:9), and MAR (RH:51) antigens are encoded by alleles at the Cc locus of the Rh system, where C
and C
are considered low-frequency antigens and antithetical to the high-frequency antigen MAR. The frequency of C
(RH:8) is approximately 2% in Caucasians, 1% in Black people, 4% in Finns, and 9% in Latvians. The aim of this study was to determine the frequency of RhD+ phenotypes in a population of southeast Brazilian blood donors and to perform a molecular study to distinguish the RHCE*Ce.08.01 and RHCE*Ce.09 alleles, responsible for the C
and C
expressions, respectively.
We investigated 11,536 RhD+ Brazilian blood donors. All samples were phenotyped for D, C, c, E, e, and C
. In the C
+ samples, a molecular analysis was performed to detect the nucleotide substitutions A122G and G106A, which determine the C
and C
antigens, respectively.
C
antigen was found in 110 (0.95%) samples in the following phenotypes: DC
e/dC
e (72/0.62%), DC
e/DC
e (30/0.26%), and DC
e/DC
E (8/0.07%). Among 110 C
+ samples, 108 showed the A122G nucleotide substitution associated with RHCE*Ce.08.01 allele and 2 samples the G106A substitution associated with the RHCE*Ce.09.01 allele.
This study showed the prevalence of the RhD+ phenotype in the Brazilian population, and that through the molecular study, it was possible to differentiate the RHCE*Ce.08.01 and RHCE*Ce.09.01 alleles. The phenotype frequency was similar from Black people (1%) and different from Caucasians, Finns, and Latvians. |
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ISSN: | 0887-8013 1098-2825 |
DOI: | 10.1002/jcla.22570 |