Real-time and quantitative PCR: applications to mechanism-based toxicology

Real-time and quantitative PCR: applications to mechanism-based toxicology

There is increasing awareness that quantitative analysis of changes in molecular targets plays a key role in addressing scientific questions in molecular toxicology, molecular epidemiology, and human risk assessment. One of the emerging technologies that is being used to analyze these molecular targ...

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Veröffentlicht in:Journal of biochemical and molecular toxicology 2001, Vol.15 (3), p.121-127
1. Verfasser: Walker, Nigel J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological Models
DNA Polymorphisms
Dose-Response Relationships
Fluorescent Dyes - metabolism
Gene Expression Profiling
Genetic Techniques
Humans
Microarrays
Models, Biological
Molecular Epidemiology
Pharmacokinetics
Polymerase Chain Reaction - methods
Risk Assessment
RT-PCR
Toxicogenomics
Toxicology - methods
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Zusammenfassung:There is increasing awareness that quantitative analysis of changes in molecular targets plays a key role in addressing scientific questions in molecular toxicology, molecular epidemiology, and human risk assessment. One of the emerging technologies that is being used to analyze these molecular targets is real‐time and quantitative (RTAQ) polymerase chain reaction (PCR). The aim of this review is to provide the reader with an overview of this technology and to highlight specific applications of this technology to some key areas of molecular toxicology. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:121–127, 2001
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.8