Preventive effect of S-allyl cysteine sulfoxide (alliin) on lysosomal hydrolases and membrane-bound atpases in isoproterenol-induced myocardial infarction in wistar rats

In this study, S‐allyl cysteine sulfoxide (SACS) was used to evaluate its preventive effect in isoproterenol (ISO)‐induced myocardial ischemia in male Wistar rats. Rats were pretreated with SACS (40 and 80 mg kg−1) orally for 5 weeks. After the treatment period, ISO (150 mg kg−1) was administered subcutaneously to rats at an interval of 24 h for 2 days. The activities of β‐D‐N‐acetyl‐glucosaminidase, β‐galactosidase, β‐glucosidase, and acid phosphatase increased in serum and heart in ISO‐induced rats. In addition, these rats showed a significant (p < 0.05) increase in the activities of β‐glucuronidase and cathepsin‐D in serum and heart and a significant (p < 0.05) decrease in their activities in lysosomal fraction of the heart. The activity of Na+K+‐ATPase declined, while those of Ca2+‐ and Mg2+‐ATPases significantly (p < 0.05) elevated in the heart of ISO‐induced rats. Pretreatment with SACS (40 and 80 mg kg−1) showed a significant (p < 0.05) effect in all the biochemical parameters studied. The effect at a dose of 80 mg kg−1 body weight was more effective than that at 40 mg kg−1 body weight and brought back all the biochemical parameters to near normal levels. Hereby, our study shows the membrane‐stabilizing as well as antioxidant effects of SACS in ISO‐induced rats. © 2007 Wiley Periodicals, Inc. J Biochem Mol Toxicol 21:118–124, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20166